羧甲基壳聚糖磁性纳米粒子的合成及应用

Synthesis and Application of Carboxymethyl Chitosan Magnetic Nanoparticles

通过合成油酸修饰的Fe3O4纳米粒子和羧甲基壳聚糖直接包埋油酸修饰的Fe3O4纳米粒子的两步合成法制备了羧甲基壳聚糖磁性纳米粒子。采用透射电子显微镜、傅里叶变换红外光谱、振动样品磁强计和同步热分析测试技术对制备的羧甲基壳聚糖磁性纳米粒子进行了表征。所得磁性纳米粒子呈规则球形,粒径约为10 nm;表面含羧基,且具有很好的顺磁性和稳定性。考察了羧甲基壳聚糖磁性纳米粒子对阿霉素的载药量和对阿霉素在磷酸盐缓冲溶液中的缓释性能。结果表明,磁性纳米粒子对阿霉素展示了较高的载药量(91.8 mg/g),结合了阿霉素的磁性复合物对阿霉素的缓释作用明显,说明制备的羧甲基壳聚糖磁性纳米粒子有望作为治疗肿瘤的纳米磁靶向药物输送载体。

Carboxymethyl chitosan magnetic nanoparticles（CMCS-OA-Fe3O4 NPs） were prepared by a two-step method,which involved,synthesizing nanosized Fe3O4 in the presence of oleic acid（OA-Fe3O4 NPs） and directly coating the OA-Fe3O4 NPs with carboxymethyl chitosan in aqueous solution.The prepared CMCS-OA-Fe3O4 NPs were characterized with transmission electron microscopy（TEM）,vibrating sample magnetometer（VSM）,Fourier transform infrared spectrometry（FT-IR） and simultaneous thermal analyzer（STA）,respectively.The CMCS-OA-Fe3O4 NPs were found to be regularly spherical with carboxyl on their surface,with a diameter of roughly 10 nm.The CMCS-OA-Fe3O4 NPs exhibited good paramagnetism and stability.The drug loading and the sustained-release property of the CMCS-OA-Fe3O4 NPs for adriamycin（ADM） in phosphate buffer solution（PBS,pH=7.4） were studied.High drug loading（91.8 mg/g） of CMCS-OA-Fe3O4 NPs and obvious sustained-release effect in vitro for ADM were reached.The results indicate that CMCS-OA-Fe3O4 NPs may be used as a magnetic-targeted nano drug carrier for the treatment of tumour.