益肾平肝方对SHR早期肾损害组织形态学的影响

Histomorphology Effects of YiShen PingGan Decoction Treating the Early Renal Damage of Spontaneous Hypertensive Rat(SHR)

目的:观察益肾平肝方对自发性高血压大鼠(SHR)早期肾损害组织形态学的影响。方法:将10周龄雄性SHR随机分为模型组(蒸馏水)、中药组(益肾平肝方)、中西药结合组(益肾平肝方+洛汀新)、西药组(洛汀新)、WKY作为正常对照组(蒸馏水),每组8只,用药12周,观察大鼠肾脏的超微结构及四型胶原(CO-Ⅳ)和层黏蛋白(LN)的表达。结果:与模型组相比,益肾平肝方能明显改善SHR的肾脏超微结构,显著降低CO-Ⅳ和LN在肾小球的表达(P<0.01)。结论:益肾平肝方可以减轻高血压病肾脏病理损伤,其作用可能是通过下调促肾小球硬化的细胞因子Co-IV、LN表达,减少ECM在肾小球局部的聚集实现的。

Object： To observe the effects of YiShen PingGan decoction on histomorphology of spontaneously hypertensive rat（SHR） with early renal damage.Methods： The 10-week-old male SHR were randomly divided into model group （distilled water）, Chinese medicine group （YiShen PingGan decoction）, the combination of western medicine group （YiShen PingGan side and Lotensin）, western group（Lotenisn）, WKY as normal control group（distilled water）, and 8 rats in each group, medicine for 12 weeks.We observed the ultrastructure o the rat liver and the expression of type IV collagen （CO-IV） and laminin （LN）.Results： YiShen PingGan decoction can improve the renal ultrastructure in SHR, decrease the expression of type IV collagen（CO-IV） and laminin（LN） in glomeruli（P〈0.01） significantly. Conclusion： YiShen PingGan decoction can reduce the hypertensive renal pathological damage, which may be achieved by down-regulating the expression of cytokines （CO- IV and laminin）promoting glomerular sclerosis, and reducing the ECM accumulation in parts of glomeruli.