猪圆环病毒2型广东株全基因组的克隆与序列分析

Cloning and Sequencing of Complete Genome of Porcine Circovirus Type 2(PCV2) Guangdong Strains

根据GenBank已发表的猪圆环病毒2型（PCV2）全基因组序列，设计2对特异性引物，对广东省不同地区规模化猪场采集的疑似断奶仔猪多系统消耗综合征（PMWS）组织病料进行了PCV2全基因组克隆和序列分析．结果表明，PCV2核苷酸序列较稳定，不同地区6个PCV2毒株全基因组序列均由1767bp组成，彼此间核苷酸序列相似性达95．3％～99．8％，亲缘关系密切；与GenBank已发表的国内不同地区PCV2参考毒株的相似性介于70．1％-99．1％；与欧洲各地区毒株的相似性介于67．7％～98．8％；其中与美国的毒株（AY094619）差异性最大，介于69．4％-70．8％之间．

According to the genomic sequence of PCV2 published in GenBankTM, two pairs of primers were designed to amplify the complete genomes of PCV2 from the tissue of pigs suspected with PMWS sampled in different districts of Guangdong Province. The results showed that the complete genome of every strain consisted of 1 767 nucleotides. Nucleotide sequence homology between the six PCV2 isolates were found to be 95.3% -99. 8% respectively. The homologies were70. 1% -99.1% compared with the sequences of PCV2 from different regions in China published in GenBank^TM ,67.7% -98.8% as compared with the sequences of PCV2 in different regions of Europe. The homologies were 69.4% -70. 8% compared with the sequence of the USA strain （AY094619） ,with which the variability was the biggest.