多重PCR法检测金黄色葡萄球菌耐药基因及致病毒素基因

Development of multiplex PCR for the detection of antibiotic-resistant gene and pathogenic genes in staphylococcus aureus

目的:了解金黄色葡萄球菌(简称金葡菌)耐药基因及所携带致病毒素基因PVL与TSST-1的特点。方法:应用多重PCR法检测mecA基因、TSST-1基因及PVL基因。结果:84株金葡球经多重PCR法对其mecA基因进行检测,检出率为58.3%(49/84)。PVL基因阳性菌株的分离率为23.8%(20/84),PVL阳性的MRSA为13株(13/49,26.5%),PVL阳性的MSSA为7株(7/35,20.0%),差异无统计学意义(P>0.05);未检出TSST-1基因。结论:金葡菌的耐药性呈上升趋势,且金葡菌可产生多种毒素,在分离金葡菌的同时,应加强其耐药基因及毒素基因的检测。

Objective： To study the features of staphylococcal toxic shock syndrome toxin （TSST-1）, PVL and antibiotic resistant gene carried by staphylococcus aureus. Methods： The detection of PVL gene, mecA gene and TSST-1 gene were carried out by multiplex PCR. Results： 84 strains of staphylococcus aureus were detected mecA gene with multiplex PCR, and the detection rate was 58.3% （49/84）. The isolation rate of PVL-positive strain was 23.8% （20/84） in S. aureus isolates. MRSA of PVL-positive had 13 strains （13/49, 26.5%）, MSSA of PVL-positive had 7 strains （7/35, 20.0%）, they showed no statistical difference （P〉0.05）. The prevalence of TSST-I gene was not detected. Conclusion： The antibiotic resistance of staphylococcus aureus is increasing. Staphylococcus aureus may secrete various toxins. We should pay attention to detecting these toxins when separating staphylococcus aureus.