苯酚和苯二酚异构体在反相色谱上的保留行为和热力学性质

Retention Behaviors and Thermodynamic Properties of Phenol and Di-hydroxybenzene Isomers on the Reversed-Phase Liquid Chromatography

在反相液相色谱中以C8色谱柱为固定相，用10％～40％甲醇为流动相，于10-50℃温度范围内研究了对苯二酚、间苯二酚、邻苯二酚和苯酚的色谱保留行为和热力学性质。结果表明：苯酚和苯二酚异构体在结构上的细微差异，能在反相色谱行为上明显地体现出来。同时还测定了苯酚和苯二酚异构体与C8色谱柱固定相之间相互作用的焓△H°和熵AS°变化情况。这些热力学参数的测定为苯酚和苯二酚异构体与疏水界面相互作用机制的研究提供了一种行之有效的方法。

The retention behaviors and thermodynamic properties of hydroquinone, resorcinol, eatechol and phenol in reversed-phase liquid chromatography are studied in a methanol concentration range of 10%-40%（v/v） and in a temperature range of 10-50 ℃. The results demonstrate that the slight different structure of dihydroxybenzene isomers and phenol can be resolved significantly in C8 hydrophobic ligand. Values for the relative changes in enthalpy（△H°） and entropy（△S°） associated with the interaction process are also determined. The determination of thermodynamic parameters will provide an alternative approach for the investigation of the interaction mechanisms of phenol and dihydroxybenzene isomers with hydrophobic surfaces.