应用系统遗传学与细胞发生的基因调控网络序列标记显示分析

Applied Systems Genetics and Sequence Tagged Fragments Display Analysis of Gene Regulatory Network Involved in Cytogenesis

基因型-表现型复杂系统自组织化是基因系统到蛋白质系统、代谢酶系统的遗传信息转换过程。一个协同表达的基因群调控一个相对独立性状的功能模块,基因网络的自组织化建构基因组稳态与遗传适应过程。腺垂体干细胞分化成5种不同的内分泌细胞系,受上丘脑和性腺、胰岛细胞等激素的调控,涉及系列转录因子的诱导表达,成为细胞系发生研究的模型。GH基因的表达受上丘脑激素GRF、GHRP-6刺激以及SMS抑制,经不同受体、G蛋白亚单元和PKA、PKC信号传导路径,转录因子调控细胞再生或GH基因表达、激素分泌。基因表达调控决定于基因序列,如启动子、非翻译RNA区、蛋白质的结构域等,系统生物学包括组学、计算与合成生物技术,序列标志片段显示(STFD),可用于细胞系分化、病理变化等基因表达谱、信号传导网络的系统分析与功能基因克隆。

The self-organization biosystem of genotype-phenotype complexity is the information transformation from gene regulatory network,proteins interaction to metabolism system. The independent phenotype which forms a functional module is controlled by synergic expression of genes group of the genotype. During evolution, networks of gene groups are constructing of genomic stability, genetic co-adaptation and self-organization of genomes. Cytogenesis of endocrine cells within pituitary is in respond to hormones stimulation, and involved in induced expression of a serial transcription factors. Cell treatments with GRF, GHRP-6, and active, inhibit PKA, PKC pathways and involved in cell proliferation and differentiation. The regulation of gene expression depends on the elements in the sequence of gene,the promoter,the un-translated region of mRNA, the membrane domain of protein etc. Systems biology includes the omics, computational and synthetic biotechnology. An efficient approach, termed sequence tagged fragments display（STFD） , be developed that allow for the identification of genes from differential expression tissues, and analysis of genes involved in cytogenesis and pathogenesis.