摘要
目的研究环境雌激素双酚A(BPA)对人卵巢癌3AO细胞、子宫内膜癌HHUA细胞及前列腺癌DU145细胞株增殖活性的影响,探讨BPA的致瘤性。方法将不同浓度的BPA以及17β-雌二醇(17β-E2)作用于3AO、HHUA及DU145细胞,并设溶剂对照,采用MTT法测定药物作用48 h的细胞增殖情况,并以浓度为10-9mol/L的BPA、10-7mol/L的E2作用于三种细胞,检测药物作用0、24、48、72 h的细胞增殖活性。结果与溶剂对照相比,BPA分别在10-5、10-9mol/L的浓度促进3AO、HHUA及DU145细胞增殖(P<0.05);10-9mol/LBPA作用48、72 h对HHUA及DU145细胞均有促增殖效应,与溶剂对照相比,差异有统计学意义(P<0.05)。结论BPA可促进激素相关肿瘤细胞3AO、HHUA及DU145的增殖,且其促进肿瘤细胞的增殖效应与剂量、时间相关。提示BPA的污染可能与激素相关性肿瘤的发生发展有关。
Objective To study the effect of bisphenol A （BPA） on the proliferation of 3AO, HHUA and DU145 cells. Methods 3AO cells, HHUA cells and DU145 cells were exposed to BPA and 17β-Estradiol （E2） in different concentrations. The proliferation activities of different cells were detected by MTr assay at 48 h. Then the absorbance values of different cells treated with 10^-9 mol/L BPA or 10^-7 mol/L E2 were obtained at 0 h, 24 h, 48 h and 72 h. Results Compared with solvent control, BPA could significantly stimulate 3AO, HHUA and DU145 ceils to proliferate in the concentration of 10^-5 ,10^-9 mol/L at 48 h （P 〈 0.05）. In addition, 10^-9 mol/L BPA could significantely promote HHUA and DU145 cells to proliferate in a time-dependent manner（ P 〈 0.05）. Conclusions The contamination of BPA might be associated with the occurrence and development of hormone-related tumors.
出处
《山东医药》
CAS
北大核心
2010年第1期9-11,共3页
Shandong Medical Journal
基金
山东省人口和计划生育委员会科学技术研究项目[(2007)年第(16)号]
