摘要
用水提-醇沉法从大蓟中提取多糖,脱脂、去蛋白,经冷冻干燥后得到粗多糖。通过苯酚-硫酸法测定糖的含量,粗多糖得率为6.5%。再采用DEAE-纤维素离子交换柱层析进一步分离纯化,得到5个次级组分DJ1,DJ2,DJ3,DJ4和DJ5。其中得率最高的是DJ2组分,为8.06‰。
The crude polysaccharide was extracted from cirsium japemicum DC. using hot waterethanol method to remove lipid and precipitate proteins. Its contents was determined by phenolsulphuric acid method after the sample was freeze-dried. The yield of the crude polysaccharide was 6.5%. The sample was further isolated into five subfractions （DJ1,DJ2,DJ3,DJ4 and DJ5） through ion-exchange chromatography of DEAE-cellulose. The highest yield is 8.06‰ from DJ2.
出处
《光谱实验室》
CAS
CSCD
北大核心
2010年第1期98-101,共4页
Chinese Journal of Spectroscopy Laboratory
基金
安徽省教育厅自然科学重点研究项目(KJ2007A123ZC)
安徽省高等学校青年教师科研资助项目(2005jq1115)
关键词
大蓟
多糖
提取
分离
离子交换柱层析
Cirsium Japonicum DC.
Polysaccharide
Extraction
Isolation
Ion-Exchange Chromatography
