FTIR光学信息研究PLD蛋白分子冻干胁迫下的二级结构变化

Study on Secondary Structural Changes of PLD Under Freeze-Dried Duress by Optical Information of FTIR

通过FTIR光学信息研究液态和固态磷脂酶D的二级结构,结果表明FTIR光学信息是一种有效的蛋白质分子二级结构研究方法。FTIR的光学信息分析表明冻干胁迫下,磷脂酶D的FTIR光学信息及二级结构发生较大变化。在磷脂酶D活性构象时的pH冻干使磷脂酶Dα螺旋大大下降,无规卷曲大大上升,β折叠几乎没有变化,说明PLD蛋白质分子空间有序构象解体严重。功能性试验表明,磷脂酶D二级结构的变化直接导致了其活性发生很大变化。

The liquid and solid PLD secondary structure were characterized by optical information of FTIR. The results showed that optical information of FTIR was an effective method for researching protein secondary structure. The optical information of FTIR shows that α-helix of PLD is greatly decreased,random coil of PLD is greatly increased and β-sheet of PLD almost is no change under freeze-dried duress when PLD is processed by freeze-drying under the condition of PLD active conformation pH. That suggested PLD protein conformational space in an orderly were disjointed seriously. The functional test show that the secondary structure change PLD is related with the activity of PLD.