摘要
应用3,5-二硝基水杨酸测定法,分析了纤维素酶水解老化纸张中纤维素物质的酶活力影响因素,根据酶活力确定了酶解反应条件。pH=4.8,温度为50℃,酶解时间为60min,加酶量为10kU/mL条件下,经纤维素酶水解后测得的还原糖量呈现一定的趋势,可以此来初步判定纸张纤维的老化程度。
Analysis of cellulase activity was studied by 3,5-dinitrosalicylic acid （DNS） method. The optimum conditions of enzymolysis were determined as follows：pH4.8,enzymolysis temperature 50℃ ,enzymolysis time 60 min ,and the enzyme solution 10kU/mL. Different aging degree of cellulose paper was determined by the decomposition of reducing sugar measured after a certain amount of trend.
出处
《光谱实验室》
CAS
CSCD
北大核心
2010年第1期107-111,共5页
Chinese Journal of Spectroscopy Laboratory
基金
公安部科技创新项目(2008YYCXLNST021)
