抑制荧光动力学法测定痕量对硝基苯胺

Inhibitory Kinetic-Spectrofluometric Determination of Trace p-Nitroaniline

在硫酸介质中,痕量对硝基苯胺对溴酸钾氧化罗丹明B荧光猝灭具有抑制作用,由此建立了抑制动力学荧光法测定痕量对硝基苯胺的分析新方法。考察了酸度、反应温度、反应时间、各种试剂等条件对抑制反应的影响。在最佳实验条件下,此方法线性范围为0.68×10-7—5.44×10-7g/mL,检出限为4.76×10-8g/mL。该方法应用于环境水样的测定,回收率为96%—107%。

A kinetic fluorimetric method was established for the determination of trace p-nitroaniline based on the inhibitory effect of p-nitroaniline on the oxidation reaction of rhodamine B by potassium bromate in sulfuric acid medium. Under the optimum experimental conditions, the detection limit and linear range of the proposed method are 4. 76×10^-8g/mL and 0. 68 ×10^-7- 5.44 ×10^-7g/mL, respectively. This method has been used to determine trace p-nitroaniline in environmetal samples with recovery of 96 %--107%.