摘要
采用凝胶渗透色谱-激光光散射联用(GPC-LS)研究了经酶解后的魔芋葡甘聚糖(KGM)重均分子量(Mw)、均方根旋转半径(Rg)、构象指数(β)与温度的相关性,并结合KGM的特性粘度拟合酶解KGM的Mark-Houwink方程。结果显示:Rg与Mw表现出近似的正相关性,且Mw、Rg及β随温度升高而逐渐增大,酶解KGM的Mark-Houwink方程为[η]=5.9×10-1Mw0.562。
The correlation between the molecular weight (Mw)of konjac glucomannan(KGM) after enzymatic hydrolysis, root mean square ratio of gyration (Rg), conformation index (β)and temperature were studied by gel permeation chromatograph-laser light scattering, and combined with the intrinsic viscosity of KGM which fitted the Mark-Houwink equation. The Rg and Mw exhibit similar positive correlation, and Mw, Rg and β inhence with the temperature. The enzymolysis of KGM follows the Mark-Houwink equation of [η]= 5.9×10^-1Mw^0.562
出处
《光谱实验室》
CAS
CSCD
北大核心
2010年第1期238-242,共5页
Chinese Journal of Spectroscopy Laboratory
基金
武汉市晨光计划(批准号:20065004116-52)
湖北省教育厅(批准号:20063036)
关键词
魔芋葡甘聚糖
分子量
激光光散射
均方根旋转半径
特性粘度
分子链形态
Konjacglucomannan
Molecular Weigh
Laser Light Scattering
Root Mean Square Ratio of Gyration
Intrinsic Viscosity
Molecular Chain Morphology