联吡啶分光光度法测蒙药利胆胶囊中微量铁

Determination of Trace Iron in Mongolian Medicine by Spectrophotometry

采用浓硝酸和高氯酸的混合消解法处理苏斯-12(利胆胶囊)样品,盐酸羟胺将铁()还原成铁(),在pH为5的条件下,用联吡啶分光光度法测定铁含量,校准曲线回归方程为:y=0.1552x-0.0014(r=0.9999)。方法的加标回收率在98.4%—101.6%之间,相对标准偏差为0.29%,苏斯-12(利胆胶囊)中铁含量为3457.76μg.g-1,该方法具有操作简单、重现性好的特点,可以运用于利胆胶囊中微量铁的测定。

Susi-12 of Mongolian（cholagogic capsule） was digested with concentrated nitric acid and perchloric acid. Fe （Ⅲ） was reduced to Fe （Ⅱ） by hydroxylammonium chloride at pH 5.0,.and determined by the spectrophotometry. The regression equation of calibration curve is y=0. 1552x- 0. 0014,and its correlation coefficient r= 0. 9999. The standard addition method was used and good recovery（98. 4%--101.6%） and RSD（0. 29%） were obtained. The result showed that iron content in sample is 3457. 76μg · g^-1. The proposed method was simple and successfully applied to the determination of trace iron in cholagogic capsule.