参附注射液对β淀粉样蛋白所致阿尔茨海默病大鼠认知行为和脑组织氧化应激的影响

Effect of Shenfu Injection on cognitive behaviour and oxidative stress in β-amyloid protein-induced Alzheimer disease rats model

目的观察参附注射液(SF)对β淀粉样蛋白(amyloid beta,Aβ)所致阿尔茨海默病大鼠认知行为和脑组织氧化应激的影响,探讨SF对阿尔茨海默病的可能治疗作用及其机制。方法30只健康SD大鼠,经过水迷宫训练后随机分为3个组(10只/组)。正常对照组:侧脑室注入蒸馏水(distilled water,DW)5μL;模型对照组:侧脑室注入Aβ5μL;Aβ+SF组:侧脑室注入Aβ5μL,腹腔注射SF(10mL/kg,每日1次)。正常对照组和模型对照组腹腔注射等量生理盐水(NS),连续14d,每日1次。2周后观察各组大鼠水迷宫潜伏期值及海马、皮质区超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的活力以及丙二醛(MDA)的表达水平。结果Aβ+SF组大鼠水迷宫潜伏期明显缩短,与模型对照组比较海马及皮质SOD、GSH-PX活力明显升高(P<0.05),MDA水平明显降低(P<0.05)。结论SF能明显增加脑组织中SOD和GSH-PX的活力,减少脑组织MDA蓄积,从而改善阿尔茨海默病大鼠认知行为。

Objective To observe the effect of Shenfu Injection（SF） on cognitive behaviour and oxyradicals in hippocampal and cortical regions of β-amyloid（Aβ） protein-induced Alzheimer disease rats model and to investigate the mechanism of Shenfu Injection for Alzheimer disease. Methods Thirty healthy SD rats was randomly separated into 3 groups（10/group） after training in water morris water maze. In blank control group,5μL of distilled water（DW） was injected into each rat via lateral ventricle. 5μL of Aβ was injected intralateroventricularly into each rat in model control group and Aβ-4-SF group. SF（10 mg/kg） was injected intraper- itoneally into each rat in Aβ＋SF group. Equal volume of normal saline was injected intraperitoneally into each rat in Aβ group and blank group. The administration lasted for 14d with once a day. Latent period value and expression levels of SOD, GSH-PX and MDA in hippocampal and cortical regions of the rats were observed at 2 weeks after the injections. Results The latent period in Aβ＋SF group was shortened significantly. The expression levels of SOD,GSH-PX in Aβ＋ SF group were higher than those in the model group（P〈0.05）. Meanwhile,the level of MDA in Aβ＋SF group decreased significantly（P〈0.05）. Conclusion SF could alleviate Aβ-induced oxidative reaction by enhancing SOD and GSH-PX activities and decrease MDA accumulation in brain tissue, which might be responsible for the behavioral improvement of Alzheimer disease rats.