顶空液相微萃取法与水蒸气蒸馏法提取金盏花挥发性成分的比较研究

Study on Volatile Oils from Calendula officinalis Prepared by Headspace Liquid Phase Micro-extraction and Steam Distillation

采用顶空液相微萃取法(HS-LPME)与水蒸气蒸馏法(SD)分别提取金盏花中的挥发性成分,经GC-MS分析,通过质谱图计算机辅助谱库检索和人工解析相结合、并参考相关文献进行化合物的定性分析,以峰面积归一化法计算各组分的相对含量。HS-LPME法所得挥发油提取物中鉴定出15种化合物,在SD法提取的挥发油中共鉴定出31种化合物,两者共有成分为14个,其中主要成分均为α-杜松醇,τ-依兰油醇,δ-杜松烯,α-依兰油烯,τ-杜松烯等,且相对含量无显著差异,5个化合物的总含量分别占总挥发油的93.43%(HS-LPME法)和87.18%(SD法)。

The volatile oils were prepared from Calendula officinalis by headspace liquid phase micro-extraction （HS-LPME） and steam distillation （SD）. Their constituents were analyzed and identified by gas chromatography-mass spectrometry combined with computer retrieval, and were quantified by calculating the relative contents basing on the peak areas normalization method. The results showed that fifteen com- ponents were identified for the volatile oil extracted by HS-LPME, and thirty-one components were identified for that extracted by SD. However, the main components obtained from both methods were found to be quite similar. Among the fourteen mutual components, α-cadinol, τ-Muurolol, τ-cadinene, δ- cadinene, α-muurolene were the main components, occupied 93.43% and 87.18% of the total volatile oils prepared by HS-LPME and SD, respectively. It was also observed that there were no significant differences in the relative contents of these five components between the two methods.