复发鼻咽癌差异基因分析及染色体定位初步研究

Identification of differentially expressed genes in recurrent nasopharyngeal carcinoma and analysis of their chromosomal location

目的通过基因芯片和生物信息学来筛选复发鼻咽癌（recurrent nasopharyngeal carcinoma，rNPC）差异基因及染色体定位和功能分析。方法选择初发鼻咽癌（primary nasopharyngeal carcinonla，pNPC）和rNPC，应用Affymetrix Genel．0ST芯片筛选差异基因；应用生物信息学对差异基因进行染色体定位和功能分析。结果差异基因共44个，其中下调基因36个，上调基因8个。基因功能分类显示，下调基因大部分属于酶活性基因（10个，27．8％），其次为钙黏合基因（7个，19．4％）、蛋白结合基因（5个，13．9％），这3类基因占了下调基因总数的61．1％，另外，受体活性基因（4个，11．1％），转录因子基因和三磷酸腺苷黏合基因各2个（各占5．6％），细胞外基质黏合基因和生长因子黏合基因各1个（各占2．8％），4个基因的功能未知（11．1％）；上调基因中，3个基因的功能未知（37．5％），酶活性基因（2个，25．O％），受体活性、钙黏合、电压门压通道调控基因各1个，各占12．5％。这些差异基因分布在大多数染色体上，主要分布在1和17号染色体上。1号染色体上包含10个基因（22．7％），17号染色体包含5个基因（11．3％）。结论差异基因分布在大多数染色体上，但主要分布在1和17号染色体上，酶活性、钙黏合及蛋白结合基因这三类差异基因可能在rNPC中扮演着重要的角色，需要进一步深入研究。

Objective To indentify differentially expressed genes in recurrent nasopharyngeal carcinoma（rNPC） by DNA microarrays, and analyze chromosomal localizations and molecular function by bioinformatics. Methods The primary nasopharyngeal carcinoma （pNPC） tissue samples and rNPC tissue samples were selected, and Affymetrix Genel. 0 ST gene chips were used to indentify differential expressed genes in rNPC, and the bioinformatics was used to analyze their chromosomal localizations as well as molecular functions. Results A total of 44 genes were identified to be differential expressed in rNPC. Thirty-six genes were down regulated, 8 genes were up regulated. Functional classification of down-regulation genes showed that most genes （10 genes,27. 8% ） belonged to the enzyme activity genes, followed by calcium ion binding genes （7 genes, 19.4% ） , protein binding genes （5 genes, 13.9% ） , receptor activity genes （4 genes, 11.1% ）, ATP binding genes （2 genes, 5.6% ）, transcription factor genes （2 genes, 5.6% ） ,extracellular matrix binding and growth factor binding have 1 gene respectively （each accounted for 2. 8%）. In addition, the functions of 4 genes （11.1%） were unknown. Functional classification of up-regulation genes showed most genes （3 genes, 37. 5% ） were unknown, followed enzyme activity genes （2 genes, 25.0% ） , receptor activity, calcium ion binding and voltage-gated ion channel activity genes have 1 genes respectively （ each accounted for 12. 5% ）. These genes were localized randomly on the most the chromosomes, with a majority of them localized on chromosomes 1, 17. Chromosome 1 contained the most differentially expressed genes （ 10, 22. 7% ） , followed by chromosomes 17 （5, 11.3% ） . Conclusions The differential expressed genes in rNPC were supposed to be randomly distributed on most chromosomes, but the majorities were found on chromosomes 1, 17. Abnormality in three groups of genes, including in enzyme activity, calcium ion binding and protein binding associate genes, might play important roles in rNPC. Those genes need to be further studied.