3种方法检测肺结核患者痰标本结果分析

Comparison of 3 Methods to Detect Sputum Specimens of Pulmonary Tuberculosis Patients

目的比较3种检测方法对肺结核患者痰菌检出率,探索噬菌体生物扩增法提高肺结核病患者结核菌阳性检出率的可行性。方法对同1例肺结核患者的痰标本,采用直接厚涂片法、结核分枝杆菌培养法和噬菌体生物扩增法检测结核分枝杆菌。结果在150例肺结核患者痰标本中,直接厚涂片法检出菌阳26例(检出率为17.3%),痰结核分枝杆菌培养阳性33例(检出率为22.0%),噬菌体生物扩增法阳性85例(检出率为56.7%),噬菌体扩增法阳性检出率高于直接厚涂片法和分枝杆菌培养法(χ2=51.8,38.8,P<0.01)。结论噬菌体生物扩增法可提高肺结核病例的痰菌检出率。

Objective To compare the detection rate for sputum specimens of pulmonary tubereulosis （TB） patients by 3 detection methods and explore the feasibility to improve the positive rate of mycobacterium tuberculosis detection by phage biological amplification. Methods The sputum specimen from one pulmonary TB pa- tient was detected by direct smear test, mycobacterium tuberculosis culture and phage biological amplification respectively. Results A total of 150 sputum specimens were detected, of which 26 were positive by direct smear test （ 17.3% ）, 33 were positive by mycobacterium tuberculosis culture （ 22% ） and 85 were positive by phage biological amphfication （56. 7% ）. The detection rate of phage biological amplification was significantly higher than the other two （ ~2 = 51.8, 38.8, P 〈 0. 01 ）. Conclusion Phage biological amplification was more sensitive and rapid in suspected TB patients differential diagnosis, so it is suitable to use in basic level laboratories.