针灸对SAMP8小鼠皮层神经干细胞增殖分化的影响

Influence of acupuncture and moxibustion on neural stem cell proliferation and differentiation in SAMP8 mice cortex

目的探讨针刺、艾灸对快速老化小鼠(SAMP8)皮层NSC增殖分化的影响。方法选用8月龄SAMP8雄性小鼠随机分为模型组、针刺组、艾灸组,以同龄雄性正常老化小鼠(SAMR1)为对照组。针刺组、艾灸组选用"百会"进行治疗,每天治疗1次,7d为1个疗程,共治疗3个疗程。对照组和模型组每天在相同时间给予相同方式的抓取。处死前1w开始给予BrdU腹腔注射,50mg/kg;治疗结束后,取皮层,用免疫荧光双标方法检测神经干细胞(NSC)增殖、分化。结果(1)各组小鼠均存在皮层NSC增殖。与对照组比较,模型组、针刺组、艾灸组阳性细胞数减少(P<0.01,P<0.05);但模型组与针刺组、艾灸组之间比较,差异无统计学意义(P>0.05)。(2)各组小鼠均存在皮层NSC分化。与对照组相比,模型组NSC分化为神经元较少(P<0.01,P<0.05)、分化为胶质细胞较多(P<0.01,P<0.05);与模型组相比,针刺组NSC分化为神经元较多(P<0.05)、分化为胶质细胞较少(P<0.01,P<0.05);艾灸组NSC分化为未成熟神经元较多(P<0.05)、分化为成熟星形胶质细胞(P<0.01)和少突胶质细胞较少(P<0.05)。结论针刺、艾灸治疗3个疗程后,主要反映在促进NSC向不同方向分化的差异。其中,针刺主要促进SAMP8皮层NSC向神经元分化,抑制向胶质细胞分化;艾灸主要是促进其向未成熟神经元分化、抑制向成熟星形胶质细胞和少突胶质细胞分化。

Objective To investigate the influence of acupuncture and moxibustion on neural stem cell（NSC） proliferation and differentiation in senescence-accelerated mouse prone 8 （SAMP8） cortex. Methods SAMP8 were randomized into model,acupuncture and moxibustion groups, SAMR1 were used as control group. Acupuncture and moxibustion therapy were applied on ＂Baihui （Du20）＂ once daily for 21 d. All the mice were given BrdU（50 mg/kg） intraperitoneal injection daily for 7 consecutive days before sacrificed; NSC proliferation and differentiation in cortex were detected by immunofluorescent double labeled staining. Results （1）NSC proliferation was founded in all groups. Compared to that of SAMR1 group, the number of NSC positive cells was decreased in SAMP8, acupuncture and moxibustion groups （ P 〈 0. 01, P 〈 0. 05, P 〈 0. 05 ） without significant difference among the latter three groups（ P 〉 0.05 ）. （2）NSC differentiation was identified in every group. The number of neurons differentiated from NSC in SAMP8 group was decreased significantly than that in SAMR1 group （ P 〈 0.01, P 〈 0.05 ） while that glial cells were increased （ P 〈 0.01, P 〈 0.05 ）. Compared to that of SAMP8 group, the number of which NSC differentiated into neurons was increased significantly （P 〈 0. 05 ） while that differentiated into glial cells was decreased （P 〈 0. 01, P 〈 0. 05 ） in acupuncture group ; the number of which NSC differentiated into immature neurons was increased significantly （ P 〈 0. 05 ） while differentiated into mature astrocytes （ P 〈 0. 01 ） and oligodendrocytes decreased （ P 〈 0. 05 ） in moxibustion group. Conclusions The diversity of NSCs differentiation are different between which accepted acupuncture and moxibustjon different stimulation. Acupuncture could pro- mote NSCs differentiated into neurons and inhibit them differentiated into glial ceils after 3 courses of treatment while moxibustion prone to promote NSCs differentiated into immature neurons and inhibit them differentiated into astrocytes and oligodendrocytes.