高效液相色谱氢化物发生原子荧光(HPLC-HG-AFS)联用技术检测海藻食品中无机砷

Detection of inorganic arsenic in seaweed food using HPLC-HG-AFS

海藻食品中无机砷的含量是水产品质量检验中主要的安全卫生指标。实验采用高效液相色谱氢化物发生原子荧光联用技术(HPLC-HG-AFS)对海藻食品中无机砷的检测进行了研究。通过实验优化了提取剂、提取时间及其仪器条件,建立了HPLC-HG-AFS联用技术检测海藻食品中无机砷含量的方法。用1.2mol/LHCl在70℃水浴下提取1h,用过氧化氢(H2O2)氧化;pH6.0的15mmol/L(NH4)2HPO4溶液为流动相,HPLC-HG-AFS上机分析。样品加标量在0.10mg/kg和1.00mg/kg时的平均回收率均在92%以上,相对标准偏差均低于4%,方法精密度较高。研究结果表明,该方法测定海藻中的无机砷较为准确、可靠,为制定农业行业标准海藻食品中无机砷的测定,提供技术支持。

The inorganic arsenic （iAs） in seaweed food is an important safety index in seafood quality detection. High performance liquid chromatography-hydride generation-atomic fluorescence spectrometry （HPLC-HG-AFS） was used to determine the iAs in seaweed food. The concentration of extraction reagents, the extraction time and instrument conditions were optimized. A new method to determine the content of inorganic arsenic in seaweed food using HPLC-HG-AFS was obtained： firstly the seaweeds were extracted with 1.2 mol/L HCl at 70 ℃ for 1 h , then the extracts were oxidized with hydrogen peroxide, and finally were analyzed by HPLC-HG-AFS under the condition of 15 mmot/L（ NH4 ）2HPO4 flow phase （ pH = 6.0）. The average recovery rates of 0.10 mg/kg and 1.00 mg/kg As Ⅲ in samples were all above 92% and the relative standard deviations were all below 4% with a high accuracy. The present study proved that HPLC-HG-AFS was a reliable method to determine the iAs in seaweed food and provide enough evidences for the establishment of the standard for iAs in seaweed food.