人绒毛膜促性腺激素对人PBMC产生MIF的影响

Effect of Human Chorionic Gonadotrophin on mRNA Expression of Human PBMC MIF

目的:探讨人绒毛膜促性腺激素(hCG)对人外周血单核细胞(PBMC)产生巨噬细胞移动抑制因子(MIF)的影响。方法:分离正常人PBMC,体外加入不同浓度的hCG,在37℃5%CO2条件下培养一定时间收获细胞或加入一定浓度的hCG,同样条件下培养,在不同时间收获细胞,用荧光定量PCR方法检测MIF mRNA转录水平。结果:在实验的hCG浓度范围内,MIF的转录水平随着hCG浓度增加而明显提高。在100U/mL的hCG作用下,MIF mRNA表达在培养的1～2h达到峰值,随后很快下降,至8h基本回到刺激前水平。结论:hCG在一定浓度范围内有促进MIF mRNA转录的作用,且在本实验筛选浓度下,MIF mRNA转录水平与作用时间相关,提示hCG可通过促进PBMC分泌细胞因子参与一定的免疫调节作用。

Objective：To investigate the effect of human chorionic gonadotrophin （hCG） on the gene expression of migration inhibitory factor （MIF） in human peripheral blood mononuclear cell （PBMC）. Methods：The healthy human PBMC was cultured with hCG at 37 ℃,5%CO2 for 2 hours. The mRNA of harvested cells was isolated. The MIF mRNA was detected by real-time RT-PCR. Results：In a certain range of doses,the mRNA expression of MIF significantly increased following the increase of hCG in a dose depandent manner,and it reached to a peak 1-2 hours after culture,then returned to the minimum level after 8 hours. Conclusion：In a certain range of doses,hCG can increase the mRNA expression of MIF. This effect is correlated with reacting time. It is suggested that hCG may involve in immune response by up-regulating the production of cytokines by PBMC.