血透患者血清对人脐静脉血管内皮细胞ICAM-1表达的影响

Effect of Serum from Maintenance Hemodialysis Patients on Expression of ICAM-1 in Human Umbilical Vein Endothelial Cells

目的:研究维持性血液透析(MHD)患者血清对体外培养人脐静脉血管内皮细胞细胞间黏附分子(ICAM-1)表达的影响及其致内皮细胞功能紊乱的机制。方法:用10%胎牛血清1640培养液(对照组,A组)、10%正常人血清1640培养液(正常人组,B组)、10%MHD患者血清1640培养液(MHD患者组,C组)分别培养人脐静脉血管内皮细胞1、3、6、12h,用免疫组织化学方法检测ICAM-1的表达情况。结果:不同血清刺激组人脐静脉血管内皮细胞ICAM-1表达的差异有统计学意义(P<0.05),与A组和B组比较,C组ICAM-1表达明显增加。不同刺激时间ICAM-1表达的差别有统计学意义(P<0.05),不同血清刺激组ICAM-1的表达与刺激时间之间存在交互效应(P<0.05)。结论:慢性肾功能衰竭MHD患者血清可促进体外培养人脐静脉血管内皮细胞ICAM-1的表达,从而导致内皮细胞功能失调。

Objective：To study the effect of serum from maintenance hemodialysis （MHD） patients on the expression of intercellular adhesion molecule-1 （ICAM-1） in human umbilical vein endothelial cells （HUVEC） cultured in vitro,and the mechanism of endothelial cells dysfunction caused by serum from MHD patients thereof. Methods：HUVEC were incubated for 1 h,3 h,6 h and 12 h in RPMI 1640 culture media containing 10 % fetal cattle serum （FCS ）（group A）,10% normal human serum （group B） and 10% MHD patient serum （group C） respectively. The immunocytochemical method was used to determine the expression of ICAM-1. Results：There were significant differences in the expression of ICAM-1 in HUVEC among stimulation groups with different serums （P〈0.05）. Compared with groups A and B,the expression of ICAM-1 was significantly increased in HUVEC of group C. There was significant difference in the expression of ICAM-1 at the different stimulation times （P〈0.05）. There was significant interaction between the expression of ICAM-1 in HUVEC and stimulation groups with different serum and the stimulation times （P〈0.05）. Conclusion：The serum from MHD patients with chronic renal failure can promote the expression of ICAM-1 in HUVEC cultured in vitro,thus causing the dysfunction of endothelial cell.