重症急性胰腺炎对胰腺亚细胞器脂质过氧化损伤及栀子的影响

Protective Effect of Gardenia Jasminoides Ellis on Lipid Peroxidation Injury of Pancreatic Subcellular Fraction Following Severe Acute Pancreatitis

目的:探讨重症急性胰腺炎对胰腺细胞线粒体和溶酶体脂质过氧化损伤及栀子的保护作用。方法:将SD大鼠经胰管逆行注射1.5%去氧胆酸钠建立重症急性胰腺炎模型,随机分为对照组、模型组和栀子治疗组,观察胰腺细胞线粒体、溶酶体超氧化物歧化酶(SOD)、丙二醛(MDA)、膜流动性以及胰腺细胞线粒体琥珀酸脱氢酶(SDH)活性、溶酶体酸性磷酸酶(ACP)释放率及栀子对上述指标的影响。结果:与对照组比较,模型组胰腺线粒体、溶酶体SOD活性、膜流动性明显降低,MDA含量增高,线粒体SDH活性下降,而溶酶体ACP释放率增加(P<0.01);栀子治疗后上述各检测指标均较模型组明显改善(P<0.01);相关分析显示MDA与SOD间存在明显负相关,MDA与膜流动性间呈显著正相关(P<0.01)。结论:栀子通过清除重症急性胰腺炎时胰腺亚细胞器氧自由基,对抗脂质过氧化,保护胰腺亚细胞器结构和功能的正常。

Objective：To explore lipid peroxidation injuries of pancreatic mitochondria and lysosome following severe acute pancreatitis （SAP） and the protective effects of gardenia jasminoides ellis （GJE） thereof. Methods：SAP models were induced by retrograde injection of 1.5% sodium deoxycholate. The rats were randomly divided into three groups：sham group,SAP group and GJE group. The changes were measured in superoxide dismutase （SOD）,malondialdehyde （MDA）,membrane fluidity of mitochondria and lysosome in pancreas and succinic dehydrogenase （SDH） in mitochondria,acid phosphatase （ACP） in lysosome.The effects of GJE were also observed. Results：Compared with Sham group,the activity of SOD and membrane fluidity of mitochondria and lysosome were decreased in pancreas,and the content of MDA were increased; the releasing rate of ACP was significantly higher in lysosome; SDH in mitochondria was lower in SAP group （P〈0.01）. The above-mentioned indexes were obviously ameliorated in GJE group than those in SAP group（P〈0.01）. The correlation analysis indicated that MDA was negatively correlated with SOD,positively correlated with membrane fluidity （P〈0.01）. Conclusion：GJE can alleviate lipid peroxidation by getting rid of oxygen free radicals of pancreatic subcellular fraction in the course of SAP,and protect the structure and function of pancreatic subcellular fraction.