音乐治疗对应激哮喘大鼠免疫功能的影响

Effects of music therapy on immune functions in asthma rats

目的观察音乐治疗对应激哮喘大鼠血清皮质醇(CORT)、肺泡灌洗液白细胞介素-4(IL-4)及脑组织白细胞介素-1β(IL-1β)水平的影响,探讨音乐治疗对应激哮喘大鼠免疫功能的影响。方法健康成年Wistar雄性大鼠32只,随机分为4组:正常对照组、哮喘模型组、应激哮喘组和音乐治疗组。用卵蛋白激发哮喘与束缚应激建立应激哮喘大鼠模型,以Mozart音乐作为缓解应激的方法。放射免疫法测定大鼠血清皮质醇、肺泡灌洗液IL-4及全脑IL-1β的含量。结果哮喘模型组IL-4含量明显高于正常对照组(P<0.05),应激哮喘组大鼠IL-4含量高于哮喘模型组(P<0.05),而音乐治疗组IL-4含量较应激哮喘组明显减少(P<0.05)。另外,应激哮喘组血清皮质醇水平较正常对照组显著升高(P<0.05),音乐治疗组与应激哮喘组比较,血清皮质醇水平明显下降(P<0.05)。应激哮喘组脑组织IL-1β含量高于正常对照组(P<0.05),其他各组无明显差异。结论心理应激加重哮喘大鼠肺组织炎性变化;音乐治疗可改善外周和中枢免疫指标,对应激大鼠的哮喘可能有一定的缓解作用。音乐治疗哮喘的可能机制是通过减少内源性皮质醇的释放,抑制Th2细胞的活化,从而减少IL-4的合成。

Objective To explore the effects of music therapy on the levels of cortisol （CORT）,interleukin-4 （IL-4） and interleukin-1β （IL-1β） in stressed asthma rats. Methods Thirty-two male Wistar rats were randomly divided into four groups：the control group,the asthma group,the stressed asthma group and the music group. Rats sensitized with ovalbumin （OVA） were exposed to OVA with a repeated restraint stress. Mozart Sonata K.448 was used to reverse the impairment of the stress. Animals were sacrificed 24 hours after the last OVA-inhalation challenge. Content of CORT,IL-4 and IL-1β was evaluated by radioimmunoassay （RIA）. Results Compared with the control group,the sensitized groups exhibited an increased IL-4 level（P〈0.05）. Music decreased the IL-4 level compared with the stressed asthma group（P〈0.05）. CORT of the stressed asthma group was higher than that of the music group and the control group（P〈0.05）. The IL-1β level of the stressed asthma group had significantly depressive changes in contrast to the controls（P〈0.05）. Conclusion Psychological stress modifies the allergic airway responses. Music therapy could improve the immunity of rats and alleviates the symptoms of asthma,which is possibly produced by reducing the release of endogenous cortisol and suppressing the activation of Th2 cells,and thus reducing the synthesis of IL-4.