摘要
目的研究Wnt/β-catenin信号传导途径在肺腺癌细胞系A549中的激活及其作用。方法用不同浓度的GSK-3β抑制剂Licl作用A549细胞,采用免疫组化法对β-catenin的细胞内表达定位,采用MTT细胞增殖实验观察细胞增殖活性,用克隆形成实验观察克隆形成能力,用Western blot检测β-catenin和干细胞分子标志OCT-4的表达。结果10mmol/LLicl作用24h,A549细胞中的β-catenin表达增加并出现核转移,同时增殖能力与克隆形成能力增强,干细胞标记蛋白OCT-4的表达增加。结论Wnt/β-catenin信号通路在维持A549细胞增殖和克隆形成能力及增强OCT-4的表达方面有着重要作用,可为肺癌治疗提供新的靶点。
Objective To study the effects of activation of Wnt/β-catenin signal transduction pathway and the potential mechanism in lung cancer A549 cell line. Methods A549 cells were stimulated with Licl in different concentrations. The position of β-catenin was realized by immunochemistry,and cell proliferation and clone formation ability were detected by MTT assay and clone formation experiment. Western-blot was used to measure expression of the stem cell marker OCT-4. Results Stimulating A549 cells with 10 mmol/L Licl for 24 h resulted in the accumulation of β-catenin and its translocation into the nucleus. Moreover,this stimuli significantly enhanced A549 proliferation by 32.24%,and increased the ability of clone formation by 100%. In addition,an up-regulation of stem cell marker OCT-4 was observed through Western-blot. Conclusion The Wnt/β-catenin signal transduction pathway plays an important role in cell proliferation,clone formation and expression of OCT-4,and may become a new target of lung cancer therapy.
出处
《山东大学学报(医学版)》
CAS
北大核心
2010年第1期74-77,81,共5页
Journal of Shandong University:Health Sciences
基金
山东省科技厅重点攻关资助项目(2006GG3202010)
关键词
信号传导
肺肿瘤
干细胞
Signal transduction
Lung neoplasms
Stein ceils