黄体酮对脑梗死大鼠脑部MMP-9、TIMP-1表达及血脑屏障通透性的影响

Effect of progesterone on the expression of MMP-9 and TIMP-1 and blood-brain barrier permeability after focal cerebral permanent ischemia in rats

目的观察黄体酮对脑梗死大鼠脑组织MMP-9、TI MP-1表达及血脑屏障通透性的影响。方法制作SD大鼠永久性局灶性脑缺血模型(permanent middle cerebral arteryocclusion,pMCAO),将大鼠随机分成假手术组、模型组、HBC溶剂治疗组和黄体酮治疗组。模型组、HBC溶剂治疗组和黄体酮治疗组,其中后3组设24h、48h、7d 3个时间点,每组每个时间点12只。应用干湿重法检测脑组织含水量;免疫组织化学法研究脑组织MMP-9、TI MP-1的表达情况。结果模型组MMP-9、TI MP-1的表达及脑水含量在各个时间点与HBC溶剂治疗组相比差异无统计学意义(P>0.05),但在黄体酮治疗组脑组织中的表达较模型组和HBC治疗组明显降低(P<0.05)。结论黄体酮可能通过调节MMP-9/TI MP-1表达,降低血脑屏障通透性,减轻脑缺血后脑水肿程度。

Objective To study the effects of progesterone on the expression of MMP-9 （matrix metalloproteinase-9, MMP-9） and TIMP-1 （tissue inhibitor of metalloproteinase-1, TIMP-1） and blood-brain barrier permeability in cerebral ischemia in rats. Methods Permanent middle cerebral arteryocclusion rats model （pMCAO） was used in this experiment. All rats were randomly divided into 4 groups： the progesterone treated group, the HBC treated group, the model group and the sham operated group. Except for shamed operated group, 12 rats were killed in each group at 24h, 48h and 7d post-occlusion, respectively. The brain water content was evaluated with dry-wet weight method. MMP-9 and TIMP-1 were measured by immunohistochemistry technique. Results In the progesterone treated group the brain water content and the expressions of MMP-9 and TIMP-1 were down-regulated compared with the model group and HBC treated group at each time point （P〈0.05）. Conclusion Progesterone may relieve cerebral edema through balancing MMP-9 and TIMP-1 after cerebral infarction.