摘要
目的研究不同比率n-6／n-3多不饱和脂肪酸饮食对模型小鼠脾脏和主动脉组织中炎症相关因子表达的影响。方法8周龄雄性apoE基因敲除小鼠分别经不同比率n-6／n-3（组1：1．28；组2：5．03；组3：9．98；组4：68．26）多不饱和脂肪酸分别饮食干预，全长主动脉经油红O染色显示动脉粥样硬化（AS）病变程度，用实时定量RT-PCR技术分析不同饮食组小鼠脾脏和主动脉中炎症相关因子的表达特征。结果随着饮食中n-6／n-3比率升高，小鼠动脉粥样硬化病变逐渐加重。与组4相比，组1，2，3饮食显著抑制AS病变（P〈0．05），并显著抑制主动脉组织中趋化因子MCP-1和黏附因子VCAM-1基因表达（P〈0．05）。与组4相比，组1饮食显著抑制脾脏促炎因子IL-6和TNFα表达（P〈0．05）。各组饮食均不影响脾脏和主动脉中抗炎因子IL-4表达（P〉0．05）。结论低比率n-6／n-3多不饱和脂肪酸（1．28～9．98）饮食可通过抑制促炎因子IL-6、TNFα和趋化因子MCP-1、黏附因子VCAM—1基因表达延缓apoE基因敲除小鼠AS的发展。
Objective To investigate the effect of varying dietary ratios of n-6/n-3 polyunsaturated fatty acids （PUFAs） on the expression of inflammatory cytokines in spleen and aorta in apolipoprotein E knockout mice. Methods The male mice were randomly assigned to four experimental groups with different ratios of n-6/n-3 PUFAs intervention （group 1： 1.28; group 2： 5.03; group 3： 9.98; group 4： 68.26） at the age of 8 week. The aortic atherosclerotic lesions were shown using Oil Red O staining. The real-time RT-PCR was used to detect the expression of inflammatory cytokines in spleen and aorta from mice fed with different diets. Results As the dietary ratio of n-6/n-3 PUFAs ascended, so did the aortic atherosclerotic lesions. Compared with group 4, the diets in group 1, group 2 and group 3 significantly suppressed the aortic atherosclerotic lesions （P 〈 0.05） and the expression of MCP-1 and VCAM-1 in aorta （P 〈 0.05）. Besides, the diet in group 1 significantly inhibited the expression of pro-inflammatory cytokines IL-6 and TNFα in spleen （P 〈 0.05 ） . However, IL-4, an anti-inflammation factor, was not influenced in spleen and aorta by the diets in all groups （P 〉 0.05）. Conclusion Low ratios of n-6/n-3 PUFAs （1.28-9.98） may postpone the development of atherosclerosis partly via inhibiting the expression of pro-inflammatory cytokines IL-6 and TNFα, chemotatic factor MCP- 1, and adhesion factor VCAM- 1.
出处
《食品与药品》
CAS
2010年第1期16-20,共5页
Food and Drug
基金
山东省教育厅科技计划项目(No.J06L11)
