鹅掌楸属种及杂种的SSR分子鉴定

Identification of Liriodendron tulipifera,Liriodendron chinense and Hybrid Liriodendron Using Species-Specific SSR Markers

以不同种源的鹅掌楸、北美鹅掌楸和不同组合的杂交鹅掌楸为试验材料,对176对SSR引物进行初筛、复筛,最后筛选出1对物种特异性SSR引物。该引物在鹅掌楸群体中特异性扩增出190bp的产物,在北美鹅掌楸群体中特异性扩增出180bp的产物,在杂交鹅掌楸群体中特异性扩增出180bp和190bp2种产物。该引物对鹅掌楸种间鉴别准确率达100%。

This paper reported that species-specific SSR markers were used to identify Liriodendron tulipifera, L, chinense and hybrid Liriodendron. Overall, 90 genotypes were taken as DNA amplification templates, of which 30 came from 20 provenances of L. chinense, 30 from 5 provenances of L. tulipifera, and 30 from 30 mating combinations of hybrid Liriodendron. Upon two round amplifying and screening, one species-specific pair primers for Liriodendron were screened from 176 pair primers. With the pair primers, a 190 bp fragment from L. chinense, 180 bp fragment from L. tulipifera, and a 190 bp and a 180 bp fragment from hybrid Liriodendron were respectively amplified. To testify the veracity of these pair primers, 10 individuals from L. chinense, 5 from L. tulipifera, and 10 from hybrid Liriodendron were used as amplification templates, and the results showed 100% veracity for species identification. Therefore, the development of the pair primers provided an effective, stable and reliable method for species-specific identification in Liriodendron at the molecular level.