感染萧氏松茎象的金龟子绿僵菌菌株的初步筛选

Preliminary Screen of Virulent Strains of Metarhizium anisopliae against Hylobitelus xiaoi

A total of 12 strains of Metarhizium anisopliae Sorokin were tested for pathogenicity to Hylobitelus xiaoi,and a superior strain was used to infect trials in a forest.The results indicated that the strain Ma1291-2 had strong pathogenicity to H.xiaoi after treatment for 30 d by dipping inoculation with (1.5±0.5)×108 conidia·mL-1.The correct mortality,the muscardine cadaver rate and the conidia content individual of H.xiaoi were respectively 70.59%,70.81% and (1.90±0.34)×108 conidia individual,and the quickest median lethal times (LT_ 50 ) were 13.89 d.The median lethal concentration (LC_ 50 ) was 1.135 3 ×106 conidia·mL-1.By infecting trials to H.xiaoi in a forest with the strain Ma1291-2,mortality of the treatment was extremely significant higher than the control,the correct mortality,the muscardine cadaver rate and the conidia content individual of H.xiaoi were 57.85%±1.19%,55.43%±2.90% and (1.12±0.41)×108 conidia individual after 30 d,respectively,and the median lethal times (LT_ 50 ) was 16.56 d.The results suggest that the strain of Ma1291-2 has great application value.

A total of 12 strains of Metarhizium anisopliae Sorokin were tested for pathogenicity to Hylobitelus xiaoi, and a superior strain was used to infect trials in a forest. The results indicated that the strain Ma1291-2 had strong pathogenicity to H. xiaoi after treatment for 30 d by dipping inoculation with （ 1.5 ± 0.5 ） × 10^8 conidia· mL^-1 The correct mortality, the muscardine cadaver rate and the conidia content individual of H. xiaoi were respectively 70.59% , 70.81% and （ 1.90 ±0.34） ×10^8 conidia individual, and the quickest median lethal times （LT50） were 13.89 d. The median lethal concentration （LCs0） was 1. 135 3 ×10^6 conidia·mL^-1. By infecting trials to H. xiaoi in a forest with the strain Ma1291-2, mortality of the treatment was extremely significant higher than the control, the correct mortality, the muscardine cadaver rate and the conidia content individual of H. xiaoi were 57.85% ±1.19% , 55.43% ± 2.90% and （ 1.12 ± 0.41） × 10^8 conidia individual after 30 d, respectively, and the median lethal times （LT50） was 16.56 d. The results suggest that the strain of Ma1291-2 has great application value.