摘要
目的:探讨蝙蝠葛酚性碱(PAMD)对胰腺癌细胞株BXPC-3移植瘤DPC4mRNA表达的影响。方法:采用右腋皮下接种胰腺癌(BXPC-3)肿瘤细胞,经腹腔注射PAMD,连续用药21d。采用荧光定量PCR(Real-timePCR)技术检测肿瘤DPC4基因表达的情况,结果:PAMD高、中、低剂量均能上调BXPC-3荷瘤小鼠瘤组织中DPC4mRNA表达量,与模型对照组比较,差异具有统计学意义(P<0.01),结论:PAMD对BXPC-3荷瘤小鼠具有明显的抑瘤作用。
Objective：research the effects of PAMD on the expression of DPC4 mRNA of wice- transplanted pancreatic cancer BXPC -3cells, Methods：Nude mouse were inoculated with pancreatic cancer BXPC -3 cells under right armpit and injected with PAMD by abdominal cavity about 21days, and DPC4 mRNA expression was detected by Real - time fluorescent quantitative PCR. Results ： showed that high, moderate, and low dose groups of PAMD could all reduce DPC4 mRNA expressions of nude mouse inoculated with pancreatic cancer BXPC - 3 cells more obviously than model control group （P 〈 0.01 ）. Conclusions ： which shows the obviously anti - tumor effect of PAMD.
出处
《中医药信息》
2010年第1期23-25,共3页
Information on Traditional Chinese Medicine
基金
2007黑龙江省自然科学基金课题(D200675)
