补阳还五汤对AD大鼠血清中胰岛素含量影响的实验研究

The empirical study of Buyang Huanwu Decoction on the influence of content of Ins in AD rats blood serum

目的:观察补阳还五汤对老年性痴呆大鼠学习和记忆能力的保护作用,并探讨其作用机制。方法:Wistar雄性大鼠50只,随机分为五组,每组10只,即正常组、假手术组、模型组、补阳还五汤组和脑复康组,采用海马区注射Aβ1-40溶液制作AD大鼠模型。各组大鼠在造模后第7d开始给药治疗,正常组、假手术组与模型组大鼠给予等体积生理盐水,连续21d。观察AD大鼠学习和记忆能力,并采用平衡饱和放射免疫分析法(RIA)检测大鼠血清中胰岛素(Ins)、胰岛素样生长因子-1(IGF-1)和胰岛素降解酶(IDE)含量的影响。结果与结论:补阳还五汤可显著降低AD模型大鼠血清中IDE的含量,提高血清中Ins、IGF-1的含量,从而改善学习记忆障碍,起到防治老年性痴呆的作用。

Objective：To observe the protection of Buyang Huanwu Decoction for learning and memory in AD rats, and to approach the mechanism of action. Methods ： Divided average five groups with fifty Wistar masculinity rats,modeled AD rats with Aβ1-40 by hippocampus injection. After seven days,administered Buyang Huanwu Decoctin to AD rats, and lasted twenty - one days. Observed learning and memory in AD rats, and used RIA method to detect the expression of Ins, IGE - 1 and IDE serum. Results ： Buyang Huanwu Decoction obviously decreased the content of IDE, increased the content of Ins and IGF - 1, and thus improved the learning and memory dysmnesy of AD rats caused by Aβ1-40.