香玲核桃离体叶片再生体系的建立

Adventitious shoot regeneration from in vitro leaf of Juglans regia cv. Xiangling

为解决核桃种质资源的实验室保存及转基因研究等问题,研究了暗培养时间、叶片放置方式及不同浓度激素组合(BA、NAA、TDZ)对香玲核桃叶片再生的影响。试验结果表明,最适宜的暗培养时间为14d;近轴面放置效果最好;不同组合的激素配比对叶片再生不定芽能力不同,最适宜香玲核桃叶片再生的培养基为:MS+BA0.5mg·L-1+NAA0.5mg·L-1+TDZ1.0mg·L-1+sucrose30g·L-1+agar6g·L-1,在该培养基上,离体叶片不定芽再生率达60%,每叶片平均再生不定芽数超过1.1个。筛选得到了香玲核桃离体叶片的最适分化及生根培养基,对离体再生培养条件进行优化,为香玲核桃的种质资源保存及遗传转化研究奠定了基础。

Experiment was conducted with the leaves of walnut cultivar Xiangling as material for studying the best medium for adventitious shoot regeneration under dark culture conditions. The result showed that the optimum dark incubation time was 14 d,and using the adaxial leaf touching medium was better for regeneration. The optimum medium was MS ＋ BA 0.50 mg·L-1 ＋NAA 0.5 mg·L-1＋TDZ 1.0 mg·L-1＋sucrose 30 g·L-1＋ agar 6 g·L-1,on this medium in vitro the adventitious shoot frequency of regeneration could reach 60%,and the shoot number per. explant was more than 1.1.