数字化色谱指纹谱技术在六味地黄滴丸药材鉴定中的应用

Chromatograph-digitized fingerprint spectrum of Liuwei Dihuang Dripping Pill

目的:采用RP-HPLC方法建立六味地黄滴丸和药材的数字化色谱指纹谱以用于该制剂中药材鉴定。方法:以马钱苷和丹皮酚为定性定量因子,采用相对保留值α为判据,建立RP-HPLC数字化指纹谱。InertsilODS-3:色谱柱;流动相是以甲醇-0.2%甲酸水溶液作梯度洗脱,流速是1mL/min;检测波长在240nm。结果:在选定的色谱条件下,建立了药材和制剂的数字化色谱-指纹谱HPLC-DFPS,提供了稳定可控的制剂指纹谱图,以及通过与空白样品比较探求了制剂中用以鉴定各药材的特征峰群。结论:结果表明利用数字化色谱指纹谱技术鉴定六味地黄滴丸中的药材是切实可行的。

AIM： To establish some HPLC-digitized fingerprint spectrum （HPLC-DFPS） in Liuwei Dihuang Dripping Pill preparation and to apply it to identification of medicinal materials. METHODS： Based on loganin and paeonoside contents adopted as qualitative and quantitative factors, relative retention value as creteria, RP- HPLC was carried out on inertsil ODS： column, mobile phase consisted of methanol-water（0.2% formyl acid）, detection wavelength was set at 240 nm. RESULTS ： Using above optimum chromatograph conditions, HPLC-DFPS of relative medicinal materials and ＂Liwei Dihuang Dripping Pill＂ preparation were established. By comparison a group of characteristic peaks could be used to identify medicinal materials. CONCLUSION： This result indicates that it is practical to use HPLC-DFPS for identification of Liuwei Dihuang Dripping Pill.