摘要
目的:从大叶白麻中提取多糖,测定多糖含量并分析其单糖组成。方法:采用热水法提取大叶白麻叶多糖,乙醇沉淀,Sevage法脱蛋白,活性碳脱色,采用蒽酮-硫酸比色法620nm处测定多糖含量,高效毛细管电泳分析其单糖组分。结果:经蒽酮-硫酸比色法测定大叶白麻叶中多糖含量为叶重的0.97%;经高效毛细管电泳分析发现,大叶白麻含至少有9种单糖,其中含量较多的为半乳糖、阿拉伯糖、甘露糖。结论:本法分离大叶白麻叶多糖在HPCE上易实现9个单糖基线分离具有灵敏度高的特点。单糖种类丰富,具有较高的研究和开发价值。
AIM:To extract and isolate polysaccharide from Poacynum Hendersonii leaves,determine its content and analyze the monosaccharide composition.METHODS:Poacynum Hendersonii leaves was extracted with hot water,crude polysaccharide was precipitated with ethanol,deproteinated according to Sevage method,coloured with acticarbon.Then of polysaccharide contents were measured by anthrone-H_2SO_4 colorimetry at the wavelength of 620 nm.The monosaccharide composition was determined by HPCE.RESULTS:The polysaccharide content was 0.97% of leaf weight,and Gal,Ara,and Man contents were three higher monosaccharides.CONCLUSION:The method is easy to carry out the baseline resolution in HPCE and has highly sensitivity.
出处
《中成药》
CAS
CSCD
北大核心
2010年第1期102-106,共5页
Chinese Traditional Patent Medicine
基金
中国科学院百人计划项目资助(0729011211)
关键词
大叶白麻
多糖
单糖
蒽酬-硫酸法
毛细管电泳
Poacynum hendersonii
polysaccharide
monosaccharide
anthrone-H_2SO_4 colorimetry
HPCE
