冰晶对黏附成骨细胞损伤的实验研究

Damage of Extracellular Ice to Attached Osteoblast Cells During Cryopreservation

低温冻结保存过程中，冰晶是造成细胞损伤的主要因素之一。本研究运用研制的方向性冰晶生长实验台，定量研究了胞外冰晶对黏附成骨细胞的损伤。结果表明，当冰晶生长方向与黏附成骨细胞中轴线的夹角在0～30度之间时，细胞存活率高达89.3±18．9％，而当它们之间的夹角在30-60度以及60-90度之间时，细胞的存率分别为49．0±19．0％，42．8±15．8％，表明细胞存活率与胞外冰晶与黏附细胞的接触面积有关。

Extracellular ice is one of the main factors that cause cell death in cryopreservation process. The damage of ice to attached osteoblast cells was quantitively studied using a directional ice formation cryostage. The results show that the cell recovery was 89.3 ±18.9% when the angle between ice forming direction and the attached cells was 0°-30°, and the recovery of cells was 49.0±19.0% and 42.8±15.8% when the angle was 30°-60° and 60°-90°, respectively. These findings confirm that the mechanical contacting area between the cells and the formed ice has influence on the recovery of cryopreserved cells.