依普拉芬对去卵巢大鼠骨力学性能的影响

Effects of ipriflavone on bone mechanical properties in ovariectomized(OVX) rats

目的研究依普拉芬对去卵巢大鼠骨密度和骨生物力学指标变化的影响。方法腹腔手术切除大鼠双侧卵巢,分为阴性对照组,依普拉芬低、中、高剂量组和雌激素对照组,另设一假手术组,分别给予基础饲料和不同剂量受试物,12周后进行骨密度和生物力学测定。结果大鼠去卵巢后骨密度显著下降,股骨的力学性能指标有较大变化。给予依普拉芬后,可使骨密度显著提高,存在一定的剂量-效应关系,弯曲强度和弯曲弹性模量明显增加,但其作用均高于雌激素对照组,差异均有统计学意义(P<0.01和P<0.05)。结论依普拉芬可增加去卵巢大鼠股骨骨密度、改善部分骨生物力学性能。

Objective To observe the effect of ipriflavone on bone mineral density（BMD） and biomechanics in ovari- ectomized（OVX） rats. Methods Rats with bilateral removal of ovaries were divided into negative control group,ipriflavone low, middle and high-dose group and the estrogen control group, setting up another sham operation group, given given basic feed and different doses of subjects materials respectively. After 12 weeks, measured the bone mineral density and biomechanical. Results The BMD of OVX group decresed significantly and femur biomechanics markers changed obviously. Ipriflavone and estrogen can increase the BMD（P 〈 0.01 ）and expresse dose-dependent manner. Flexural strengh and flexural elastic module increase, when after given ipriflavone the bone density were significantly improved,there was a dose-response relationship, the bending strength and bending modulus increased significantly, but lower than estrogen control group, the differences were statistically significant （ P 〈 0.01 ,P 〈 0.05 ）. Conclusion Ipriflavone and estrogen can increase the BMD and improve bone biomechanics.