不同绵羊品种BMPR-IB基因多态性与产羔数的关系研究

The Relationship between Polymorphism of BMPR-IB Gene and Litter Size in Different Sheep Breeds

以BMPR-IB基因为候选基因,采用PCR-RFLP方法测定了小尾寒羊和无角道赛特及其杂一代的基因型,并对该位点不同基因型与产羔数之间的关系进行了研究。结果表明,小尾寒羊BB、B+、++基因型频率分别是30.0%、66.0%、4.0%;无角道赛特只有++型为1.0;道寒F1代没有BB型,B+和++的基因型频率分别为72.0%和28.0%。经x2检验,小尾寒羊的基因型和产羔数的关系密切,样本在该位点未达到Hardy-Weinberg平衡状态,基因型++、B+、BB的品种平均窝产羔数分别为1.00±0.00、1.67±0.11、2.40±0.13,差异极显著(P<0.01)。

Bone morphogenetic protein receptor IB （BMPR-IB） gene, which was detected in small tailed han sheep, poll dorset sheep and their hybrid F1 generation sheep （small tailed han sheep x poll dorset sheep, hereafter be shorted as Han-dorset F1） by PCR-RFLP, as a candidate gene, and the relationship between different genotypes and litter size in BMPR-IB gene was analyzed in this paper. The results indicated that the genotype frequency of BB, B＋ and ＋＋ of small tailed han sheep were 30.0%, 66.0% and 4.0%, respectively; only ＋＋ genotype was detected in poll dorset sheep, and its genotype frequency was 1.0; BB genotype was not detected in Han-dorset FI, the genotype frequency of B＋ and ＋＋ were 72.0% and 28.0%, respectively. The relationship between genotype and litter size in han sheep was verified by X^2, and the results revealed that this locus was out of Hardy-Weinberg balance. Average litter size of ＋＋, B＋ and BB were 1.00±0.00, 1.67±0.11 and 2.40＋0.13, respectively, and the diffe000000000000000rence was extremely significant （P〈0.01）.