高直链玉米淀粉对聚己内酯组织工程支架结构和性能的影响

Effect of High-Amylose Corn Starch on Structure and Properties of Poly(ε-Caprolactone) Tissue Engineering Scaffolds

采用高直链玉米淀粉改性聚己内酯(PCL),通过溶剂浇铸/粒子沥滤法制备淀粉分/聚己内酯(SPCL)组织工程支架;通过傅里叶变换红外光谱、X射线衍射、动态接触角测试及细胞相容性试验等考察了改性后支架材料的链结构、结晶性、亲水性和细胞相容性.结果表明:适量淀粉的引入能够降低支架材料的结晶性,改善材料的亲水性和细胞相容性;淀粉含量为30%的SPCL支架材料的细胞粘附率、增殖率和细胞活性最佳,有望应用在骨组织工程中。

By using high-amylose corn starch to modify poly （ε-caprolactone） （PCL）, starch/poly （ε-caprolactone） （SPCL） tissue engineering scaffolds were prepared via the solvent casting/particulate leaching. Then, the molecular chain structure, crystallinity, hydrophilicity and cell compatibility of the modified scaffolds were investigated by means of FT-IR, XRD, dynamic contact angle measurement and cell compatibility test. The results show that the addition of starch with proper dosage may decrease the crystallinity and improve the hydrophilicity and cell compatibility of the scaffolds, and that the scaffold with a starch dosage of 30% is of the best cell adhesion, proliferation and activity;, which promises to be applied to bone tissue engineering.