顶空毛细管气相色谱法测定头孢他美钠中4种有机溶剂的残留量

Determination of Residual Organic Solvents in Cefetamet by Capillary Gas Chromatography with Headspace Sampling

采用顶空毛细管气相色谱法,测定了头孢他美钠中乙醇、丙酮、二氯甲烷和四氢呋喃四种溶剂的残留量.用DB-624毛细管气相色谱柱顶空进样法,FID检测器.乙醇、丙酮、二氯甲烷和四氢呋喃的线性范围分别为0.2～7.0mg·mL^-1（r=0.9995）,0.2～7.0mg·mL^-1（r=0.9994）,0.03～1.0mg·mL^-1（r=0.9993）,0.02～0.9mg·mL^-1（r=0.9994）;乙醇、丙酮、二氯甲烷和四氢呋喃的平均回收率分别为100.5%、99.6%、99.0%、98.4%;RSD（n=5）分别为1.2%、2.3%、1.8%、2.8%.本方法简单、准确、灵敏度高、重现性好,适用于头孢他美钠中有机溶剂残留量的测定.

Headspace gas chromatography method was used for the determination of four kinds of residual organic solvents in cefetamet. The samples were injected into DB-624capillary column by headspace sampler and analyzed with FID detector. The linear ranges of ethanol, acetone ,dichloromethane and tetrahydro furan were 0.2-7.0 mg·mL-1 （r=0.9995）,0.2-7.0 mg·mL-1 （r=0.9994） ,0.03-1.0 mg·mL-1 （r=0.9993） and 0.02-0.9 mg·mL-1 （r=0.9994） respectively. The recoveries were 100.5 %,99.6 %,99.0 % and 98.4 % with RSD 1.2 %, 2.3 %,1.8 % and 2.8 %（n=5） respectively.The method is simple, accurate and sensitive with good reproducibility. It can be used for the determination of the residual organic solvents in eefetamet.