金线莲有效成分的降血糖作用及其机制的研究

Hypoglycemic effects of anoectochili active components and its mechanism

目的研究金线莲有效成分AR5对四氧嘧啶诱导的高血糖小鼠的降血糖作用,并初步探讨其降血糖作用的机制。方法将高血糖小鼠随机分为5组:模型对照组、苯乙双胍阳性对照组、有效成分低剂量(50mg.kg-1)、中剂量(100mg.kg-1)和高剂量(150mg.kg-1)治疗组,同时设正常对照组,给药组连续灌胃给药3天后,测定血清葡萄糖、胰岛素、甘油三酯和总胆固醇的含量。结果有效成分低、中、高3个剂量组均显著降低高血糖小鼠的血糖(P<0.05);中、高剂量组均显著降低血清甘油三酯含量(P<0.05);高剂量组显著提高血清胰岛素水平和降低总胆固醇含量(P<0.05)。结论金线莲有效成分可显著降低四氧嘧啶诱导的高血糖小鼠的血糖。金线莲增加胰岛素水平,调节脂代谢的作用,可能与其降血糖的作用有关。

OBJECTIVE To investigate the hypoglycemic effect of Anoectochili active components Ar5 on alloxan-induced hyperglycemic mice and its mechanism. METHODS For the study of single compound of Anoeetoehili, the hyperglycemic mice were randomized to five groups： no treatment group, phenformin group, low dosage（50mg· kg^- 1 ）, middle dosage（ 100mg· kg^- 1 ） and high dosage（ 150g ·kg^- 1 ） groups, and normal mice were set up as normal control group. Fasting serum glucose, insulin, total Cholesterol （TC）, triglyceride （TG） were measured after 3 successive days treatment. RESULTS The mean levels of serum glucose in the three At5 groups were markedly reduced（P 〈 0.05）.Also, the level of triglyceride were significantly reduced in both middle and high dosage groups of Ar5;and moreover, the level of total Cholesterol was significantly decreased and the level of insulin was increased in high dosage of Ars group（P 〈 0.05）. CONCLUSION Anoectochili effective component decreased serum glucose in Alloxan-induced hyperglycemic mice. Those Anoectochili increased serum insulin level and improved lipid profiles could contribute to its hypoglycemic effect.