实时荧光PCR法检测脑出血患者ApoE基因多态性的意义及探讨

The significance and exploration of detecting the polymorphism of ApoE Gene for patients with cerebral hemorrhage by Real-time Fluorescence Quantitative PCR

目的探讨载脂蛋白E(ApoE)基因多态性与脑出血的关系及实时荧光聚合酶链反应(PCR)技术在ApoE基因多态性检测中的应用价值。方法对福建附一医院住院的74例脑出血患者进行研究,正常对照组为80例本院健康体检者;ApoE基因型采用实时荧光PCR方法检测,血清TC、TG、LDL、HDL在全自动生化仪上采用动力学法检测。结果脑出血患者组ApoEε4等位基因频率为14.9%,明显高于正常对照组(P<0.01);比较不同ApoE基因型脑出血患者的血清TC、TG、LDL、HDL含量,结果ε4组的TC含量明显高于ε3和ε2组(P<0.05,P<0.01),LDL含量明显高于ε3和ε2组(P<0.05)。结论ApoEε4等位基因可能是脑出血的易感因子和风险因素;实时荧光PCR技术检测ApoE基因多态性具有方便、快捷的优点,值得推广。

OBJECTIVE To investigate the relation between the polymorphism of ApoE gene and Cerebral Hemorrhage and the value of Real-time Fluorescence Quantitative PCR in detecting the polymorphism of ApoE gene.METHODS Seventy-four patients with Cerebral Hemorrhage hospitalized in Fujian Provincial hospital were studied.Normal control group were eighty healthy cases in our hospital.ApoE genotype was detected by Real-time Fluorescence Quantitative PCR.Serum TC.TG.LDL.HDL were detected by dynamics method in automatic biochemical analyzer.RESULTS The allele frequency of ApoEε4 in patients with Cerebral Hemorrhage group was 14.9%,which was obviously higher than Normal control group（P〈0.01）.Serum TC.TG.LDL.HDL content were compared in different patients with Cerebral Hemorrhage＇s ApoE genotype.the TC content in group ε4 was obviously higher than group ε2 and ε3（P〈0.05,P〈0.01）.LDL content was obviously higher than group ε2 and ε3（P〈0.05）.CONCLUTION The allele frequency of ApoEε4 maybe the susceptible factor and risk factors.Detecting the polymorphism of ApoE Gene by Real-time Fluorescence Quantitative PCR is convenient and rapid,worthing to be popularized.