摘要
利用前期研究建立的一套水稻品种DNA指纹检测技术体系,对2002—2006年浙江省主要的98个水稻品种、以及2007—2008年155个浙江省区试水稻品种(181个次,含26个续试品种)进行了DNA指纹检测,构建了279个次水稻品种×12个微卫星标记的DNA指纹图谱数据库。系统分析了指纹库中各标记的多态性和品种的相似性。基于构建的指纹数据库,对所有品种的特异性及区试续试品种年度间的一致性进行了鉴定。结果发现5组11个品种组内品种间特异性不明显,1个续试品种年度间鉴定结果不一致。
A total of 98 major rice varieties in Zhejiang Province from 2002 to 2006 and 155 rice varieties in Zhejiang provincial regional trial from 2007 to 2008 were assayed with a set of DNA fingerprint testing system on rice variety.A DNA fingerprint database containing genotypic data of 279 rice variety times 12 marker loci was constructed.The polymorphism of different markers and genetic similarity of varieties in the DNA fingerprint database were analyzed.Based on the DNA fingerprint database,the distinctness of all rice varieties and consistency of the same varieties in different years were identified.It was found that 11 varieties of 5 groups had no obvious distinctness within groups,and discrepancy existed in the same variety in different years.
出处
《浙江农业学报》
CSCD
北大核心
2009年第6期555-560,共6页
Acta Agriculturae Zhejiangensis
基金
浙江省"三农五方"科技协作项目(Sn200606)
中央级公益性科研院所专项资金项目(2006RG002)
关键词
水稻品种
微卫星标记
DNA指纹库
特异性
rice variety
microsatellite marker
DNA fingerprint database
distinctness
