间歇性高糖对大鼠肾小管上皮细胞NRK-52EA转分化的影响

Effects of Fluctuating High Glucose on the Transdifferentiation of Tubule Endothelial Cell Line NRK-52EA in Mouse

目的:探讨间歇性高糖诱导大鼠肾小管上皮细胞株NRK-52EA转分化干预作用。方法:NRK-52E细胞分为空白组、正常葡萄糖组、高糖组、间歇性高糖组,分别作用72 h后,Western blot检测转化生长因子β1(TGF-β1)、Ⅰ型胶原、金属蛋白酶-2(MMP-2)、α平滑肌肌动蛋白(α-SMA)以及甲状旁腺素相关肽(PTHrP)的表达;CM2H2DCFDA试剂盒检测活性氧(ROS)含量。结果:高糖组及间歇性高糖组NRK-52E细胞TGF-β1、Ⅰ型胶原、MMP2、α-SMA以及PTHrP表达均上调;间歇性高糖组TGF-β1、MMP2以及α-SMA表达水平较高糖组高(P均<0.05),其ROS含量也显著高于高糖组(P<0.01)。结论:间歇性高糖能通过氧化应激诱导肾小管导管上皮细胞表达TGF-β1、MMP2,并促进NRK-52EA细胞转分化为α-SMA细胞。

Objective To investigate the effects of fluctuating high glucose on the transdifferentiation of tubule endothelial cell line NRK-52EA in mouse.Methods NRK-52EA cells were divided into control group,glucose group,high glucose group,fluctuating high glucose group,the expression of transforming growth factorβ1（TGF-β1）,typeⅠcollagen,MMP-2,α-SMA and PTHrP were detected with Western blot,and the content of ROS was determined with CM2H2DCFDA kits after treated for 72 h.Results The expression of TGF-β1,typeⅠcollagen,MMP-2,α-SMA and PTHrP were all up-regulation in high glucose group and fluctutating high glucose group;the expression of TGF-β1,MMP-2,α-SMA and the content of ROS in fluctutating high glucose group was significantly higher than that of high glucose group（P0.05 or P0.01）.Conclusion Fluctuating high glucose could induce the up-regulation of the expression of TGF-β1,MMP-2 in tubule endothelial cells through oxidative stress,and promote the transdifferentiation from NRK-52EAb to α-SMA.