延龄草组织培养研究

Research on the Propagaton of Trillium tsehonoskii M.in Vitro

目的：探索延龄草Ｔｒｉｌｉｕｍｔｓｅｈｏｎｏｓｋｉ组织培养繁殖技术，为保护和利用这一珍贵药用植物提供新的技术途径。方法：以ＭＳ培养基为基本培养基，附加不同浓度的ＢＡ，ＩＡＡ，ＮＡＡ，玉米素，对不同器官，不同取材时期的延龄草外植体进行愈伤组织诱导和芽分化研究。结果：ＢＡ和ＩＡＡ有利于延龄草愈伤组织的诱导。生长旺盛的根茎是较适宜的培养器官，其愈伤组织诱导频率达２１９％，且较容易分化根茎而形成植株。结论：组织培养繁殖延龄草是一种可行的、具有应用潜力的生物技术方法。而如何进一步提高愈伤组织分化小根茎的频率，是建立延龄草组培繁殖技术的关键。

Objective:To develop a technique of propagating Trillium tsehonoskii in vitro as new way to conserve and utllize this special medicinal plant.Method:Senments from different organs of the plant sampled at defferent growth stages were cultured on MS medium supplemented with various combinations of BA,IAA,NAA and zeatin in a set of concentrations.Result:The effects of the induction of callus and differentiation of sprouts were investigated,showing that BA and IAA favored the formation of callus.The stock segments taken in the period of rapid growth of the plant were suitable for tissue culture.The induction rate of callus from the stock segments was as high as 21.9% and easy to differentiate microstock and piantlets,which may result from an activate function of stock cambium to enhance cell division during the stage of sprouting to rapid growth.Conclusion: The tissue cultrue method is a practicable and potential biotechnological way to multiply Trllium tsehonoskii plants.One key point to establish an in vitro system is to further improve the plantlet differentiation rate of the callus induced.