摘要
比较了8种稀释液在藏獒精液冷冻保存和低、常温保存(5℃,10℃,15℃,20℃)的效果。结果表明:2号和8号稀释液在冷冻保存时效果最好,精子活力和顶体完整率分别达到0.40和49%,与对照组相比差异显著(P<0.05)。在藏獒的低温、常温保存试验中,所有稀释液在15℃时保存时间最长,综合考虑,7号液保存效果最好;在15℃时精子平均存活时间为103 h,显著高于对照组和其它稀释液(P<0.05)。
The Tibetan mastiff semen preservation extenders in different temperatures were selected to compare the semen quality of 8 different extenders in this experiment. The results show that the extender of No. 2 and No. 8 were the best for diluting semen in the cryopreservation tests, the spermatozoa progressive motility is 0.40 and the percentage of intact aerosome is 49% ,and the difference was significant( P 〈0.05 ). Compared with the control group, the liquid semen could survive longer at the temperature 15℃ than any other groups( P 〈0.05 ) ,and the extender No. 7 was the best for diluting semen in liquid semen preservation tests,the average survival time of sperm was 103 h,there was significant difference compared with control group and other groups( P 〈0.05 )
出处
《家畜生态学报》
2009年第6期72-75,共4页
Journal of Domestic Animal Ecology
关键词
藏獒
稀释液
精液保存
Tibetan mastiff
extender
semen preservation