阴道毛滴虫致病株可溶性抗原的电泳分析

ELECTROPHORETIC ANALYSIS OF SOLUBLE ANTIGENS OF A PATHOGENIC ISOLATE OF TRICHOMONAS VAGINALIS *

目的：研究阴道毛滴虫一致病分离株的全虫可溶性抗原。方法：应用ＳＤＳ－ＰＡＧＥ、激光光密度扫描、免疫印迹法等，对阴道毛滴虫一致病虫株的全虫可溶性抗原进行分析。结果：ＳＤＳ－ＰＡＧＥ分离出１４条蛋白带，在所分离的可溶性抗原中，分子量≤７２ｋＤａ的占８９．５％，分子量为４８－５１ｋＤａ的占１９．２％，＞１００ｋＤａ的占７．４％，最低分子量为１３ｋＤａ。免疫印迹试验显示免疫反应具有广泛性分子量范围，但在８６ｋＤａ附近未显示出免疫反应。结论：在阴道毛滴虫一致病虫株全虫可溶性抗原中，分子量为４８ｋＤａ蛋白质具有较高的蛋白含量和较强的免疫学反应。

AIM: To analyze the parasite soluble antigens of a pathogenic isolate of Trichomonas vaginalis. METHODS: The soluble antigens of a pathogenic isolate of T.vaginalis isolated from a patient suffered from trichomonad vaginitis was studied by means of SDS PAGE followed by densitometric analysis and immunoblotting. RESULTS: A total of 14 distinct protein bands was demonstrated using a 10% resolution gel concentration. Densitometrogram indicated that the soluble antigens with MWs of equal to and below 72 kDa accounted for 89 5% of the total volume with the dominant fractions of 48-51 kDa which accounted for 19 2%. Having MWs over 100 kDa accounted only for 7 4%, while the lowest M.W.fraction demonstrated was found to be at the site of 13 kDa. By immunoblotting, the specific anti T.vaginalis antibodies raised in mice recognized a broad spectrum of molecular size range of these antigenic fraction. However, no immunological reaction was seen at the 86 kDa site. CONCLUSION: The proteins of MWs 48-51 kDa possess a larger protein content and a higher immunological reactivity.