追氮时期对两种穗型小麦氮素代谢及蛋白质组分的影响

Effects of Nitrogen Topdressing Stage on N Metabolism and Protein Compontents in Winter Cultivars with Two Spike-types

在大田条件下,研究了追氮时期对两种穗型冬小麦品种氮素的积累、运转以及蛋白质组分的影响。结果表明,拔节期追氮显著增加开花期和成熟期小麦植株氮素积累量及转运量,而抽穗期追氮反而下降,氮素转运率和贡献率则随追氮时期后移而降低。兰考矮早八GS活性和豫麦49-198 NR活性在所有测定日期均表现随追氮时期后移而增加,兰考矮早八NR和豫麦49-198的GS活性在灌浆前期呈现与此相同趋势,而在灌浆后期则以拔节期追氮最高。推迟追氮时期,两品种籽粒总蛋白、清蛋白、醇溶蛋白和谷蛋白含量均增加,而球蛋白却降低。兰考矮早八剩余蛋白含量随追氮时期后移而降低,豫麦49-198则相反。

The field experiment was carried out to investigate the effects of nitrogen topdressing stage on nitrogen accumulation,transfer,assimilation enzymes and protein compontents in two wheat cultivars with different spike types.The results indicated that nitrogen topdressing at jointing stage increased N accumulation at anthesis and maturity and N transfer compared with nitrogen topdressing at turn green stage and heading.N translocation efficiency and N contribution proportion decreased with nitrogen topdressing stage delaying.GS activity of flag leaves in Lankaoaizao8 and NR in Yumai 49-198 increased with the nitrogen topdressing stage delaying,and NR activity of flag leaves in Lankaoaizao8 and GS in Yumai 49-198 took on above change rules in early filling with maximum of nitrogen topdressing at jointing stage in late filling.Albumin,gliadin,glutenin and total protein content increased with the nitrogen topdressing stage delaying,and globulin content decreased.The other protent content in Lankaoaizao8 decreased with the nitrogen topdressing stage delaying,and increased in Yumai 49-198.