水通道蛋白4mRNA在大鼠蛛网膜下腔出血后脑水肿中的表达及尼莫地平对其表达的影响

Aquaporin-4 expression in rat brain edema after subarachnoid hemorrhage and effect of Nimodipine on aquaporin-4 expression

目的:研究大鼠蛛网膜下腔出血后脑组织AQP4mRNA的表达及脑含水量的变化以及应用尼莫地平对AQP4mRNA表达的影响。方法:健康成年大鼠60只,随机分为对照组、假手术组、SAH组、尼莫地平组,建立蛛网膜下腔出血模型,分别在6h、1d、3d、5d、7d个时间点断头取脑,测定脑含水量,并用RT-PCR法检测AQP4mRNA表达。结果:SAH组脑含水量及脑组织内AQP4在6h后即增加,随时相递增,3d达到高峰,较假手术组及尼莫地平组明显增高(P<0.05)。结论:大鼠蛛网膜下腔出血后脑水肿的程度与AQP4的表达成正比,这表明AQP4在蛛网膜下腔出血性脑水肿的病理生理中起着非常重要的作用。尼莫地平可以下调AQP4mRNA的表达,从而减轻脑水肿的程度。

Objective：To study the AQP4 expression, brain water content and effect of Nimodipine on the expression. Methods： Sixty normal Wistar rats were divided randomly into four groups： control group, sham operation group, subarachnoid hemorrhage group and Nimodipine group. The changes of the brain water content and AQP4 expression were determined by RT-PCR after subarachnoid hemorrhage at 6h, ld,3d,5d and 7d. Results：The brain water content and AQP4 expression of the subarachnoid hemorrhage group increased at 6h, the two rising with time increasing,and reached peak at 3d,obviously higher compared with the sham operation group and Nimodipine group （P〈0.05）. Conclusion： The expression of AQP4 is positively related to brain edema degree after subaraehnoid hemorrhage, suggesting that AQP4 may play an important role in the pathophysiology of brain edema after subarachnoid hemorrhage. Nimodipine can downregulate the expression of AQP4 and the degree of brain edema.