HIF-1α和MMP-2在胃低分化腺癌中的表达及浸润转移的关系

Expression of hypoxia inducible factor-1α and matrix metalloproteinase-2 in poorly differentiated gastric adenocarcinoma and their relationship with invasion and metastasis

目的:研究缺氧诱导因子-1α(HIF-1α)与基质金属蛋白酶-2(MMP-2)在胃低分化腺癌中的表达,并探讨二者在胃低分化腺癌浸润转移过程中的相互关系及作用。方法:采用S-P免疫组化方法,对胃低分化腺癌石蜡标本54例及正常胃组织20例,每例切片两张,分别检测HIF-1α和MMP-2的表达情况,结果进行统计学处理。结果:胃低分化腺癌组织中HIF-1α、MMP-2阳性表达明显高于正常胃组织(P<0.05),二者与胃低分化腺癌的浸润深度、淋巴转移、TNM分期均显著相关(P<0.05),HIF-1α与MMP-2的表达呈正相关(r=0.580,P<0.05)。结论:HIF-1α和MMP-2在胃低分化腺癌组织中的表达呈正相关(r=0.580,P<0.05),表明二者在胃低分化腺癌的侵袭转移过程中存在一定的协同作用。二者可能成为判断预后的指标。

Objective：To investigate the expression of hypoxia inducible factor-1α （HIF-1α） and matrix metalloproteinase-2 （MMP-2） in poorly differentiated gastric adenocarcinoma （PDGA） and their relationship with tumor cell invasion and metastasis. Methods：We detected the expression of HIF-1α and MMP-2 in the paraffin-embedded specimen of 54 cases of poorly differentiated gastric adenoearcinoma （two slices for one case） and 20 cases of normal gastric tissue by immunohistochemistry. The results were analyzed statistically. Results：The positive expression rates of HIF-10. and MMP-2 were significantly higher in PDGA than those in the normal gastric tissue,with a significant difference between the two （P 〈0.05） . HIF-1α and MMP-2 was obviously correlated with the depth of invasion, lymph node metastasis and TNM stage of PDGA （P 〈 0.05）, and there was a positive correlation between HIF- 1α expression and MMP- 2 expression （r= 0. 580, P〈 0.05）. Conclusion ：A positive correlation between the HIF-1α. expression and MMP-2 expression in PDGA suggests both have a certain synergy during the invasion and metastasis of PDGA and both could be indicators of prognosis.