摘要
本研究在本实验室成功构建的奶牛乳房炎抗性相关cDNA文库的基础上,旨在通过对牛β-防御素基因的分析,进一步揭示乳房炎抗性的分子机理。以牛β-防御素BNBD5基因作为乳房炎抗性的候选基因,通过克隆该基因的编码区,成功构建了毕赤酵母表达载体pPICZαA/BNBD5,并采用毕赤酵母表达系统表达了BNBD5蛋白。该结果为今后BNBD5抗体的制备奠定了基础。
Based on the peripheral blood leukocyte cDNA library constructed by suppression subtractive hybridization（SSH） in our lab,bovine β-defensin 5 gene（BNBD5） was chosed as one of the candidate genes.The aim of the current research was to look for molecular events and establish fundamental materials and techniques for further study.Finally,the coding sequence（CDS） of BNBD5 gene was successfully amplified.The Pichia pastoris expression vector was constructed and the BNBD5 protein was expressed.This research lays the foundation for preparation of antibodies of BNBD5.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第1期123-126,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家科技支撑计划课题(2006BAD04A01)
国家自然科学基金(30571329)资助
关键词
奶牛
乳房炎
Β-防御素
毕赤酵母
表达
dairy
cow mastitis
β-defensin
Pichia pastoris
expression
