布鲁氏菌的脂肪酸分型研究

Typing on the cellular fatty acids of Brucella species by Gas-chromatography analysis

目的探索脂肪酸分型方法对布鲁氏菌进行分型的可能性。方法选择90株布鲁氏菌经化学方法提取菌体脂肪酸后,用气相色谱分析,获得布鲁氏菌脂肪酸成分的数据资料。并利用SPSS11.5统计软件对获得的数据资料进行聚类分析。结果布鲁氏菌脂肪酸分型方法将90株布鲁氏菌分为5组:第1组为部分牛种菌;部分羊种菌;部分猪种菌;部分绵羊附睾种;部分变异牛种;部分变异羊种;沙林鼠种标准株。第2组为猪种1、2、3、5型标准株和疫苗株S2;羊种疫苗株M28和Rev.1;绵羊附睾种标准株。第3组为部分羊种;部分变异羊种菌;部分牛种3型和6型;犬种;部分绵羊附睾种。第4组为犬种菌标准株。第5组为部分羊1型;部分变异羊种;部分牛1型;部分猪1型和3型。结论依据布鲁氏菌菌体脂肪酸含量差异可以对布鲁氏菌进行分型;依据19∶0CYCLOω8c,18∶1ω7c,16∶0三种脂肪酸含量差异可以区分猪种布鲁氏菌和犬种布鲁氏菌;脂肪酸分型结果进一步提示犬种布鲁氏菌不只1个生物型;脂肪酸分型结果进一步证实牛3型和牛6型布鲁氏菌高度同源。

To investigate the the possibility to utilize the cellular fatty acid（CFA）information as a method in Brucella typing,90 Brucella strains were subjected to the study on CFAs,and all the experimental strains were inoculated on Brucella Agar plates for 48 hours.After that,cells were harvested,saponificated,methylated and extracted to provide fatty acids methylesters for gas chromatography analysis.Based on the CFAs data matrix,dendrogram of 90 experimental strains was generated by SPSS11.5 software package.As shown in the dendrogram,90 Brucella strains could be divided into 5 clusters.The first cluster included some species of Brucella abortus,Brucella melitensis,Brucella suis,Brucella ovis;and some of the variant strains of Brucella abortus and Brucella melitensis and the typical strain of Brucella neotomae.The second cluster included typical strains of Brucella suis（1,2,3 and 5 types）;vaccine strains of Brucella suis S2;vaccine strains of Brucella melitensis M28、Rev.1 and typical strain of Brucella ovis.The third cluster included some of Brucella melitensis;some of the variant strains of Brucella melitensis;some of Brucella abortus（3,6 types）;Brucella canis and Brucella ovis.The fourth cluster was the typical strain of Brucella canis.and the fifth cluster included some of Brucella melitensis（1 type）;some of Brucella abortus（1 type）;some of the variant strains of Brucella melitensis and Brucella suis（1,3 type）.It is apparent that CFAs information can be used in brucella typing.and Brucella suis and Brucella canis can be distinguished by the difference in the CFA contents of 3 fatty acids 19∶0CYCLOω8c,18∶1ω7c and 16∶0.The results of CFAs typing in Brucella species show that Brucella canis includes 2 biovars at least and the high homologization of Brucella abortus（3 type）and Brucella abortus（6 type）can be found.